A capacity field of 20 four-year-olds will compete for the $20 million on the line in the 2023 Golden Eagle (1500m) at Rosehill Gardens.
Race favourite Amelia's Jewel has drawn perfectly in barrier five in Saturday afternoon's feature event in Sydney.
The Simon Miller-trained mare will be looking to bounce back from her worst performance of her career, where she finished ninth in the Toorak Handicap (1600m) at Caulfield on October 14.
Damian Lane will continue his association with the superstar from Western Australia in what will be Amelia's Jewel's first run in Sydney.
Following the barrier draw, horse racing betting sites took no risks and tightened her in to a +300 favourite.
Having competed in The Everest (1200m), the Gai Waterhouse & Adrian Bott-trained Hawaii Five Oh (+400) will jump from barrier four on Saturday.
The son of I Am Invincible finished midfield in The Everest and will be ridden by the returning Nash Rawiller from barrier four.
Chris Waller's Kovalica (+700) copped a horror draw (16), in his attempt to claim victory for the first time since the Group 1 Queensland Derby (2400m) in May.
Kovalica went down by a nose in the Group 1 Epsom Handicap (1600m), before finishing fifth, beaten 4.48 lengths behind Fangirl in the Group 1 King Charles III Stakes (1600m).
Following an arrogant win in the Listed Matamata Cup (1600m), Kiwi mare Legarto (+700) is back on Australian shores for the first time since her win in the Group 1 Australian Guineas (1600m).
Japan will be represented by Obamburumai (+1000), with the Japanense runner set to be partnered by Joshua Parr, following the withdrawal of star jockey Yutaka Take.
Obamburumai will jump from barrier 2 on Saturday.
Saturday's Golden Eagle will be the fifth rendition of the race, first won in 2019 by Kolding for trainer Chris Waller and jockey Glen Boss.